Molecular identification of Mutations causing resistance to tetracycline in mycoplasma isolated from patients with multiple sclerosis (Ms) in Kerman province

Background: Bacterial infections on the initiation or escalation, play a role in MS, and reports of the involvement of mycoplasma with worldwide distribution in these patients‏.‏ Mycoplasma with the host cell membrane to mimic the autoimmune attack and the other macrolide antibiotics have become resistant to the first treatment. The purpose of this research was to identify mutations causing resistance to tetracycline in mycoplasmas isolated from patients with MS Kerman province.

Methods: A total of 32 samples of cerebrospinal fluid and 48 samples of urine results in a non-random sampling of patients with MS prepared and enrich one night in PPLO broth, were gathered during a 7 weeks of continuous cultivation in PPLO broth and agar. DNA was extracted from positive examples to identify the type of bacteria (Nested-PCR) and was used tetracycline resistant (rrs3 and rrs4) of 16SrRNA the method (Duplex-PCR), as well as was performed nucleotide sequencing and phylogenetic tree.

Results: Growth was observed in 12 cases, but after (Nested-PCR), only 5 samples were detected (1 sample of cerebrospinal fluid and urine samples 4) mycoplasma. But In (Duplex-PCR), to show only urine samples, alleles‏.

Conclusion: The phylogenetic was found to have mutated alleles at different points, but for a definitive answer to the resistance to tetracycline, additional research is required. Based on the findings, have mutated alleles has the potential to tetracycline resistance.