Comparative Clinicopathological Study on pH and Haemoglobin of Blood

Aim: Aim of this study to compare the pH and hemoglobin of blood on different genders and different age groups.

Introduction: The full form of pH is the potential of hydrogen, pH is known as the negative logarithm of H+ ion concentration. The pH of any components of body, including blood, is indicated on the pH scale. The value of pH ranges from 0 to 14. A pH value of 7.0 is considered as neutral. Blood is slightly basic; the normal pH level of blood is 7.35 to 7.45. Usually, the body maintains the pH of blood close to 7.40. Hemoglobin is a protein molecule that helps the RBC to transport oxygen. The term heme reforms to the iron-containing porphyrin. This iron-containing hemoglobin is responsible for the red color of the red blood cells.

Materials and Methods: 15 blood samples were collected from the lab in Saveetha Dental College and Hospitals. The low sample size is one of the limitations. The pH value is measured by using a multi reagent strip, hemoglobin level was measured by using an automated counter machine, and these values were plotted. The pH value was tabulated and the data were analyzed with SPSS version (23.0).

Results: Mean hemoglobin value was observed different in different age group. In age groups of 21-30 years, the value was 12.95g/dl, in age groups 31-40 years the value was 12.08g/dl, in age groups of 41 to 50 years the value is 13.20g/dl and for age group more than 50 years the value is 14.05g/dl. Mean pH value on comparing with age, the value of pH was 7 for the age group of 21-30 years, 31-40 years was 7, 41 to 50 years was 7.04 and for more than 50 years was 7. Correlation analysis reveals no significant relationship between hemoglobin and pH with increasing age in both males and females.

Conclusion: The value of hemoglobin is increased among different age groups and the pH level remains the same and on comparing with gender the hemoglobin level increases with age group and pH remains the same.