Analysis of Fluorescently Labeled ISSR Markers to Reveal the Genetic Diversity of Cassava Varieties (Manihot esculenta Crantz) in the Kerala’s Tribal Area of India

The study Inter Simple Sequence Repeat (ISSR) molecular markers to genotype distinct cassava (Manihot esculenta Crantz) cultivars within tribal communities in Kerala, India. Fluorescent ISSR primers were labelled with 6-carboxyfluorescein and used for PCR amplification. The total number of bands produced was 72, of which 49 were found to be polymorphic markers; an average of 17 polymorphic bands was obtained per primer. The manuscript is a scientific validation of the traditional knowledge of tribal people regarding the diversity and wealth of cassava crops in the region, and this validation can therefore help in protecting the farmers right on a particular landrace that they cultivate. The technique used in the diversity analysis is a time-saving process as compared to conventional breeding programs for crop improvement. The data in the manuscript also supports similarity and differences of each variety to one another, which is analysed statistically with the molecular marker-based data generated. By examining genetic diversity at a high resolution, ISSR markers can provide valuable insights into the evolutionary dynamics of these cassava populations. This research contributes to the broader goal of enhancing agricultural sustainability and food security in tribal communities.