Biobutanol Production from Pineapple Peels Waste using Single and Co-Culture of Clostridium acetobutylicum and Clostridium beijerinckii Isolated from Keffi Metropolis

Aims: This study investigated the production of biobutanol from pineapple peels using single and co-cultures of locally isolated strains of Clostridium acetobutylicum and Clostridium beijerinckii.

Methodology: Soil samples were collected from ten different areas in Keffi. Clostridium species were isolated using standard methods and identified using standard molecular techniques involving the use of 16S rRNA and compared with type strains.

Results: The yield of biobutanol in this study ranged from 1.2±0.20g/L to 5.6±0.43g/L in single culture fermentation broth and 5.8±0.37g/L to 6.5±0.51g/L in co-culture fermentation broth.

Conclusion: It was observed that in single culture and co-culture, the fermentation broth of Clostridium acetobutylicum (SAA1) produced the highest yield of biobutanol in comparism with the yield of other isolates.