Detection of Drug-resistant S. aureus from Poultry Samples Collected from Different Areas of Bangladesh

Akhi, Mahmuda Akhter and Das, Nantu Chandra and Banik, Avijit and Abony, Maruf and Juthi, Muniara and Uddin, Muhammad Ekhlas (2019) Detection of Drug-resistant S. aureus from Poultry Samples Collected from Different Areas of Bangladesh. Microbiology Research Journal International, 29 (1). pp. 1-10. ISSN 2456-7043

[thumbnail of Banik2912019MRJI51324.pdf] Text
Banik2912019MRJI51324.pdf - Published Version

Download (251kB)

Abstract

Staphylococcus aureus is gram-positive cocci that can cause foodborne illness which can be transmitted by chicken meat, both raw and undercooked; consumption of which may cause infection and/or toxicity in consumers. This current study was conducted for the detection of the prevalence of S. aureus in three types of poultry samples which included chicken meat, chicken eggs and droppings. Samples were aseptically collected from different rural and urban areas of 8 districts of Bangladesh in triplicate collection method which was conducted in the Centre for Excellence, Department of Microbiology, Primeasia University, Dhaka, Bangladesh to detect the Multi Drug-Resistant (MDR) S. aureus. Isolation was done by using 7.5% Sodium Chloride broth for enrichment and Mannitol salt agar after enriching samples in Buffer Peptone water. Antibiogram was done by using Kirby Bauer Method on Muller Hinton agar. The current study revealed 68% (17/25), 84% (21/25) and 52% (13/25) of S. aureus in chicken meat, eggs and dropping, respectively. Around 75.47% isolates (40/53) showed 100% similarity with S. aureus from the generated Dendrogram based on biochemical data. Cefixime (62.67%) showed the highest sensitivity against all of the isolates while Penicillin (86.62%) exhibited the highest resistance. Moreover, 59.62% of isolates were resistant to 5 or more drugs (Multidrug-Resistant). Poultry products such as meat and eggs were contaminated with drug-resistant S. aureus which can cause serious health effect to the consumer. The drug-resistant pathogen in droppings helps to indicate the spread of drug-resistant isolates in the environment. Therefore, proper sanitation measures should be taken to ensure the quality of the products during slaughtering, collecting eggs or disposing of poultry litter.

Item Type: Article
Subjects: Afro Asian Archive > Biological Science
Depositing User: Unnamed user with email support@afroasianarchive.com
Date Deposited: 01 May 2023 07:11
Last Modified: 28 Aug 2024 14:09
URI: http://info.stmdigitallibrary.com/id/eprint/382

Actions (login required)

View Item
View Item