Preservation and Transportation of Hepatocyte Monolayers in A No Spill Format at Non Freezing Temperatures

Evans, Peter J. (2014) Preservation and Transportation of Hepatocyte Monolayers in A No Spill Format at Non Freezing Temperatures. International Journal of Biology, 6 (2). pp. 58-63. ISSN 1916-9671

[thumbnail of 32821-116041-1-PB.pdf] Text
32821-116041-1-PB.pdf - Published Version

Download (1MB)

Abstract

Hepatocyte monolayers were preserved under a gelatin overlay at 10°C. Morphological changes were observed after 4 days, as structure less areas at the cell periphery. The changes could be prevented by adding sucrose (0.05M) to the gel. Cell viability remained high for 7 days. Cell spreading occurred rapidly when the cells were reactivated. Basal enzyme levels were maintained during the preservation period. The hormonal induction of tyrosine aminotransferase was stable for 4 days but declined subsequently. Sucrose addition extended the period of preservation where induction was evident. The method provides a robust system that allows preservation in the absence of potentially deleterious chemicals. Time is allowed to send cells anywhere in the World in a no spill format whist arriving at their destination as if freshly produced.

Item Type: Article
Subjects: Afro Asian Archive > Biological Science
Depositing User: Unnamed user with email support@afroasianarchive.com
Date Deposited: 23 May 2023 06:47
Last Modified: 24 Jul 2024 09:46
URI: http://info.stmdigitallibrary.com/id/eprint/824

Actions (login required)

View Item
View Item