Evans, Peter J. (2014) Preservation and Transportation of Hepatocyte Monolayers in A No Spill Format at Non Freezing Temperatures. International Journal of Biology, 6 (2). pp. 58-63. ISSN 1916-9671
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Abstract
Hepatocyte monolayers were preserved under a gelatin overlay at 10°C. Morphological changes were observed after 4 days, as structure less areas at the cell periphery. The changes could be prevented by adding sucrose (0.05M) to the gel. Cell viability remained high for 7 days. Cell spreading occurred rapidly when the cells were reactivated. Basal enzyme levels were maintained during the preservation period. The hormonal induction of tyrosine aminotransferase was stable for 4 days but declined subsequently. Sucrose addition extended the period of preservation where induction was evident. The method provides a robust system that allows preservation in the absence of potentially deleterious chemicals. Time is allowed to send cells anywhere in the World in a no spill format whist arriving at their destination as if freshly produced.
Item Type: | Article |
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Subjects: | Afro Asian Archive > Biological Science |
Depositing User: | Unnamed user with email support@afroasianarchive.com |
Date Deposited: | 23 May 2023 06:47 |
Last Modified: | 24 Jul 2024 09:46 |
URI: | http://info.stmdigitallibrary.com/id/eprint/824 |